Fig. 3.

Two Rab GAPs display impairments in DCV release similar to rab-5 and rab-10 mutants. (A) The Rab GAP mutants, tbc-2 and tbc-4, have severe defects in DCV release. Error bars indicate SEM. ***P < 0.001 (one-way ANOVA with Bonferroni post test). (B) Schematic representation of the domain structure of TBC-4. CC, CC domain; TBC, tre-2/cdc16/Bub2 domain. (C and D) Full-length TBC-4 interacts with RAB-8 and RAB-11.1 (C) in a GTP-dependent manner (D). A catalytically inactive mutant of TBC-4 (R155A) specifically interacts with RAB-10 in a GTP-dependent manner. (E) RAB-8 and RAB-11.1 interact with an N-terminal CC domain of TBC-4 (1–100 aa). (F) TBC-4 and RAB-10 localize to the same compartments in VNC neurons. (Scale bar: 4 μm.)