C5a and ICs induce LTB4 and IL-1β release from resting PMNs. (A) Dose–response analysis for the release of LTB4 from PMNs after stimulation with varying concentrations of murine recombinant C5a for 1 h. The EC50 of LTB4 induction by C5a is 25.4 ng/mL. (B) Release of LTB4 from PMNs after stimulation with IC, C5a (10 ng/mL), or both combined for 1 h. (C) Release of IL-1β from PMNs after stimulation with IC, C5a (100 ng/mL), CCL3 (100 ng/mL), CXCL2 (100 ng/mL), and IFN-γ (10 ng/mL) alone or combined for 18 h. All data represent concentrations in the supernatant determined by ELISA, shown as mean ± SD (n = 3 independent experiments). *P < 0.05, **P < 0.01, ***P < 0.001 compared with unstimulated control, ###
P < 0.001 compared with stimulation with IC, and +P < 0.05 compared with stimulation with C5a.