Fig. 3.

5-LO/C5aR but not 5-LO/FcRγ coexpression in radiosensitive cells is required for arthritis. (A and B) Arthritis in WT → WT, Fcer1g^−/− → WT, Alox5^−/− → WT, Alox5^−/−/Fcer1g^−/− → WT, Alox5^−/−/WT → WT, and Fcer1g^−/−/WT → WT BMC (n = 3–4 mice per group; one representative of two independent experiments shown). P < 0.001 in clinical score and AT for WT → WT, Alox5^−/−/Fcer1g^−/− → WT, Alox5^−/−/WT → WT, or Fcer1g^−/−/WT → WT vs. Fcer1g^−/− → WT, or Alox5^−/− → WT. (C) Histological score of ankles from mice in A and B (n = 9–15 mice per group; data compiled from three independent experiments). *P < 0.05, **P < 0.01, and ***P < 0.001 for indicated group vs. Alox5^−/− → WT; ^#P < 0.05, ^##P < 0.01, and ^###P < 0.001 for indicated group vs. Fcer1g^−/− → WT. (D) Respective representative sections. (E and F) Arthritis in WT → WT, C5ar^−/− → WT, Alox5^−/− → WT, Alox5^−/−/C5ar^−/− → WT, Alox5^−/−/WT → WT, and C5ar^−/−/WT → WT BMC (n = 4–5 mice per group; one representative of three independent experiments is shown). P < 0.001 in clinical score for WT → WT, Alox5^−/−/WT → WT, or C5ar^−/−/WT → WT vs. Alox5^−/− → WT, Alox5^−/−/C5ar ^−/− → WT, or C5ar^−/− → WT; P < 0.001 in AT for WT → WT or Alox5^−/−/WT → WT vs. Alox5^−/− → WT, Alox5^−/−/C5ar^−/− → WT, or C5ar^−/− → WT; and P < 0.01 in AT for C5ar^−/−/WT → WT vs. Alox5^−/− → WT, Alox5^−/−/C5ar^−/− → WT, or C5ar^−/− → WT. (G) Histological score of mice from A and B (n = 9–15 mice per group; compiled from three independent experiments). ***P < 0.001 for indicated group vs. Alox5^−/−/C5ar ^−/− → WT; ^##P < 0.01, ^###P < 0.001 for indicated group vs. Alox5^−/− → WT; ⁺⁺P < 0.01, ⁺⁺⁺P < 0.001 for indicated group vs. C5ar^−/− → WT. (H) Respective representative sections. All data are presented as mean ± SEM. (Scale bars, 100 μm.)