Microcalorimetric titrations of native and mutant McpS-LBR with malate. (Upper) Raw titration data of McpS-LBR, McpS-LBR R60A, and McpS-LBR R254A. Protein was at 33 μM, and aliquots of 1 mM malate were injected. (Lower) Integrated, concentration-normalized, and dilution heat-corrected raw data of the titration of McpS-LBR with malate.