Fig. 4.

Acyl chain type and presence of cholesterol influence liposome binding of other retroviral Gag proteins. The three Gag proteins were expressed in a reticulocyte lysate and analyzed by flotation as in Fig. 1. RSV, Rous sarcoma virus (alpharetrovirus genus); MLV, murine leukemia virus (gamma retrovirus genus). The lipid compositions were as follows: 0% = 70% egg PC/30% brain PS; 36% = 34% egg PC/30% brain PS/36% cholesterol (white bars); Ld = 10% DSPC/30% POPS/50% POPC/10% cholesterol; Lo = 22% DSPC/30% DPPS/23% POPC/25% cholesterol. The histograms represent at least three replicas; error bars show SD. Order parameter (S), as determined via ESR, is 0.156 for the Ld composition and 0.332 for the Lo composition.