Table 1.
Gene | Enzyme | Diacylglycerol acyltransferase activity (in vitro) | Influence on lipid bodies/TAG accumulation | M. leprae homologue | M. leprae homologue-diacylglycerol acyltransferase activity (in vitro) | Reference |
---|---|---|---|---|---|---|
Rv3130c (tgs1) | DGAT | + | Δtgs1decreases TAG accumulation | ML1244 | ND | [26, 78] |
Rv3734c (tgs2) | DGAT | + | ND | ML1244 | ND | [26, 78] |
Rv3234c (tgs3) | DGAT | + | ND | ML1244 | ND | [26, 78] |
Rv3088 (tgs4) | DGAT | + | ND | ML1244 | ND | [26, 78] |
Rv1760 | DGAT | + | ND | ML1244 | ND | [26, 78] |
Rv2285 | DGAT | + | ND | ML1244 | ND | [26, 78] |
Rv3804c (85A) | DGAT | + | Overexpression increases production of lipid bodies | ML0097 (85A) | ND | [79] |
BCG1489c [Rv1428c] | DGAT | ND | ΔBCG1489c reduces TAG accumulation | ML2427c | ND | [80] |
BCG3153c (tgs1) [Rv3130c] | DGAT | ND | ΔBCG3153c reduces TAG accumulation | ML1244 | ND | [77] |
BCG3794c (tgs2) [Rv3734c] | DGAT | ND | ΔBCG3794c reduces TAG accumulation | ML1244 | ND | [80] |
| ||||||
Gene | Enzyme | TAG-hydrolyzing activity (in vitro) |
Influence on lipid bodies/TAG accumulation | M. leprae homologue | M. leprae homologue-TAG-hydrolyzing activity (in vitro) | Reference |
| ||||||
Rv3097c (lipY) | Lipase/esterase | + | ΔlipY reduces TAG hydrolysis. Overexpression increases TAG hydrolysis |
ML0314c (lipU) ML1053 ML1183c |
ND | [76] |
Rv1399c (lipH ) | Lipase/esterase | + | ND | ML0314c (lipU) | ND | [75] |
BCG1721 [Rv1683] | Lipase/esterase | + | + | ML1346 | ND | [80] |
Rv0183 | Lipase/esterase | Hydrolyzes only monoacylglycerides |
ND | ML2603 | ND | [81] |
Rv2224c | Lipase/esterase | ND | ND | ML1633c | ND | [82] |
| ||||||
Gene | Enzyme | Isocitrate cleavage (in vitro) |
Influence on lipid bodies/TAG accumulation | M. leprae homologue | M. leprae homologue-isocitrate cleavage (in vitro) | Reference |
| ||||||
Rv0467 (icl) Rv1916 (icl2) |
isocitrate lyase | + | ND (but the Δicl, Δicl2 strain shows no intracellular replication) | ML1985c (aceA) | ND | [83–87] |