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. 2012 Oct 22;1(10):e31. doi: 10.1038/oncsis.2012.31

Figure 1.

Figure 1

Expression of WNT3-Myc and FZD7-EE in HepaRG and OUMS-29 cells. (a) Immunostaining showing protein expression of WNT3 (Myc-Tag, green) and FZD7 (EE-Tag, red) in RG-W3/F7- and OU-W3/F7-stable-expressing clones. RG-EV and OU-EV were used as negative controls ( × 400). (b) Expression of WNT3 and FZD7 mRNAs by qRT–PCR normalized to 18S rRNA expression. Bar graphs depict a ratio to the controls OU-EV or RG-EV. Stable clones expressing single WNT3 (W3) or FZD7 (F7) were used as positive controls. *P<0.01 compared with the negative controls OU-EV or RG-EV.