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. 2012 Oct 22;1(10):e31. doi: 10.1038/oncsis.2012.31

Figure 4.

Figure 4

Activation of the WNT3/FZD7-mediated signaling promotes cell migration. (a, b) Analysis of cell migration in HepaRG (a) and OUMS-29 (b) expressing both WNT3 and FZD7 as measured by a wound-healing assay. Top: Representative photographs taken at 0 h, 12 h and 24 h post-wound ( × 40). Bottom: The wound closure was quantified at 8 h, 12 h and 24 h post-wound by measuring the remaining unmigrated area using ImageJ. (c) Transwell migration assay. Left: Quantification of the number of migratory cells using the transwell assay. Migratory cells were counted in 10 non-overlapping frames of the membrane using Stereologer (Dissector, Newton, MA, USA). Right: Representative photographs of stained cells attached to the bottom membrane of a transwell ( × 100). *P<0.01 compared with the negative controls OU-EV or RG-EV.