Skip to main content
. 2012 Oct 15;1(10):e29. doi: 10.1038/oncsis.2012.30

Figure 5.

Figure 5

PPA-induced cell death through targeting the methylosome. (a) H1299 cells were transfected with pcDNA3/FLAG-PPA or pcDNA3/FLAG. Cell lysates were immunoprecipitated with a FLAG antibody conjugated gel. Precipitation with a normal mouse IgG plus a protein G conjugated agarose served as a control. The precipitated complexes were examined by western blotting analysis with antibodies against MEP50 and PRMT5. Actin, MEP50 and PRMT5 in whole-cell lysates was monitored for expression levels. (b) H1299 cells were transfected with a control siRNA or PRMT5 siRNA followed by a second transfection with pEGFP-PPA-C1 after 48 h. Bars show 100 μm. (c) H1299 cells were transfected with a control siRNA or PRMT5 siRNA. After 48 h, the whole-cell lysates were examined by western blot with antibodies against PRMT5 and actin.