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ThT fluorescence profile of Aβ(25–35) time-course-aggregation. (a) Aβ(25–35) concentration in HFIP during the crio-lyophilization was 224 μM. Aβ(25–35) (100 μM) was incubated in phosphate buffer (34.5 μM, pH 7.4), with 17.5% of acetonitrile at RT. (b) Aβ(25–35) concentration in HFIP during the crio-lyophilization was 1.793 mM. Aβ(25–35) (50 μM) was incubated in phosphate buffer (34.5 μM, pH 7.4), with 17.5% of acetonitrile at RT.
