Effect of peptide concentration
and pH on Aβ(25–35)
aggregation: ThT fluorescence profile. (a) Aβ(25–35)
(50, 100, and 200 μM) was incubated in phosphate buffer (34.5
μM, pH 7.4), with 17.5% of acetonitrile at 4 °C; (b) Aβ(25–35)
(100 μM) was incubated in phosphate buffer (34.5 μM, pH
5, 7.4 and 8), with 17.5% of acetonitrile at 4 °C.