Table 2. Effect of Treatment with CPT-cAMP for 6 h on Protein Expression of WT and kin- S49 Cells^a.

	WT CPT 6 h:WT ctrl
gene symbol	accession number	average ratio	unique_pep	spectrum_num	protein
PKA-Dependent
Coenzyme Metabolic Process
Idh3g	IPI00109169	4.43 (0.55*)	2	22	isocitrate dehydrogenase [NAD] subunit γ
Gsr	IPI00760002	4.28	3	30	glutathione reductase
Glyr1	IPI00817029	2.18	2	4	glyoxylate reductase 1 homologue
Chromatin Modification
Morf4l2	IPI00463666	2.46	2	10	mortality factor 4-like protein 2
Huwe1	IPI00453615	0.57	4	27	isoform 4 of E3 ubiquitin-protein ligase
Nasp	IPI00115987	0.32	2	17	nuclear autoantigenic sperm protein isoform 1
Protein Transport
Gdi2	IPI00122565	2.02	3	17	isoform 1 of Rab GDP dissociation inhibitor β
Ran	IPI00134621	1.56	2	72	GTP-binding nuclear protein Ran
Nasp	IPI00115987	0.32	2	17	nuclear autoantigenic sperm protein isoform 1
Ptges3	IPI00762817	5.45	2	5	prostaglandin E synthase 3
Tsnax	IPI00137296	4.20	2	17	translin-associated protein X
Capza2	IPI00111265	2.45	2	9	F-actin-capping protein subunit α2
Rpsa-ps7	IPI00677305	2.25	7	287	similar to 40S ribosomal protein SA
Dcps	IPI00459280	2.10	2	13	scavenger mRNA-decapping enzyme
Exosc4	IPI00310857	1.89	2	35	exosome complex exonuclease RRP41
Pgam5	IPI00226387	1.86	2	16	isoform 2 of phosphoglycerate mutase family member 5
Gm4492	IPI00853739	1.64	4	65	similar to gag
Anp32e	IPI00277026	1.64	4	121	isoform 2 of acidic leucine-rich nuclear phosphoprotein 32 family member E
Ppia	IPI00554989	1.60	4	34	peptidyl-prolyl cis–trans isomerase
Anxa5	IPI00317309	0.47	2	8	Annexin A5
Cops4	IPI00131871	0.46	2	7	signalosome complex subunit 4
Lrrc59	IPI00123281	0.46	2	17	leucine-rich repeat-containing protein 59
Uchl5	IPI00124938	0.45	2	11	isoform 1 of ubiquitin carboxyl-terminal hydrolase isozyme L5
Tnpo1	IPI00798527	0.22	4	12	Tnpo1 transportin-1
PKA-Independent
Exosc7	IPI00387489	0.60 (0.46*)	2	12	exosome component 7
Thy1	IPI00109727	0.40 (0.5*)	2	14	Thy-1 membrane glycoprotein
Psme2	IPI00124225	0.24 (0.3*)	3	13	Psme2b-ps proteasome activator complex subunit 2

^aProteins whose levels of expression change in WT or kin- S49 cells incubated with CPT-cAMP for 6 h are listed. The proteins are classified according to the DAVID bioinformatics software. The data shown are the average ratios (WT CPT 6 h:WT ctrl or kin-CPT 6 h:kin- control cells) from at least two independent experiments. The proteins whose levels of expression increased and decreased upon treatment are shown in bold and italics, respectively. Also shown are the number of unique peptides identified for each protein (unique_pep) and of PQD spectra (spectrum_num) used for protein quantitation. The PKA-independent proteins (i.e., whose expression changes in the kin- cells following a 6 h incubation with CPT-cAMP) have two numbers in the average ratio column. The ratios upon CPT-cAMP treatment for 6 h in kin- cells (kin- CPT 6 h:kin- Ctrl) are shown in parentheses.