Table 3. Effect of Incubation with CPT-cAMP for 24 h on Protein Expression in WT S49 Cells^a.

	WT CPT 24 h:WT ctrl
gene symbol	accession number	average ratio	unique_pep	spectrum_num	protein
Regulation of Cell Cycle
Calr	IPI00123639	1.90	4	206	CalrCalreticulin
Ywhah	IPI00227392	0.56	3	38	Ywhah 14-3-3 protein η
Cep192	IPI00762607	0.41	2	63	Cep192 40 kDa protein
ATP Binding
Skiv2l2	IPI00460846	4.82	2	6	Superkillerviralicidic activity 2-like 2
Galk1	IPI00265025	3.09	3	9	galactokinase 1
Zap70	IPI00331216	2.63	2	35	tyrosine-protein kinase ZAP-70
Map2k1	IPI00466610	1.67	4	18	dual-specificity mitogen-activated protein kinase kinase 1
Ruvbl2	IPI00123557	1.60	2	20	RuvB-like 2
Vars	IPI00130353	1.60	8	153	valyl-tRNA synthetase
Mthfd1	IPI00122862	0.56	3	21	C1-tetrahydrofolate synthase, cytoplasmic
Cellular Response to Stress
Supt16h	IPI00120344	1.88	4	8	FACT complex subunit
Ruvbl2	IPI00123557	1.60	2	20	RuvB-like 2
Pcna	IPI00113870	0.60	4	142	proliferating cell nuclear antigen
Protein Transport
Pcna	IPI00113870	0.60	4	142	proliferating cell nuclear antigen
Ywhah	IPI00227392	0.56	3	38	14-3-3 protein η
Rpl38	IPI00677340	0.55	2	19	similar to ribosomal protein L38
Regulation of Transcription
Anp32a	IPI00314736	2.93	2	30	acidic leucine-rich nuclear phosphoprotein 32 family member A
Supt16h	IPI00120344	1.88	4	8	FACT complex subunit
Pdlim1	IPI00309768	1.86	2	22	PDZ and LIM domain protein 1
Ruvbl2	IPI00123557	1.60	2	20	RuvB-like 2
Ywhah	IPI00227392	0.56	3	38	14-3-3 protein η
Zinc Ion Binding
Calr	IPI00123639	1.90	4	206	calreticulin precursor
Pdlim1	IPI00309768	1.86	2	22	PDZ and LIM domain protein 1
Cpsf3	IPI00648606	0.64	2	33	Cpsf3 protein
Lgals7	IPI00468187	5.89	3	19	lectin, galactose binding, soluble 7
Gsr	IPI00760002	4.34	3	30	cytoplasmic of glutathione reductase
Ptges3	IPI00762817	4.04	2	5	prostaglandin E synthase 3
Ndrg1	IPI00125960	3.63	2	37	protein NDRG1
Dcps	IPI00459280	2.29	2	13	scavenger mRNA-decapping enzyme
Hint1	IPI00108189	2.19	3	17	histidine triad nucleotide-binding protein 1
Diap1	IPI00831596	2.15	6	57	diaphanous homologue 1
Hnrpab	IPI00117288	1.93	3	110	heterogeneous nuclear ribonucleoprotein A/B
Tpi1	IPI00467833	1.73	6	230	triosephosphate isomerase
BC018371	IPI00351472	1.66	5	123	hypothetical AMP-dependent synthetase- and ligase-containing protein
Dbi	IPI00108756	0.54	2	15	acyl-CoA-binding protein
AK122209	IPI00605125	0.50	2	6	hypothetical protein LOC382038
Acat2	IPI00228253	0.36	2	62	acetyl-CoA acetyltransferase
Exosc7	IPI00387489	0.28	2	12	exosome component 7

^aProteins whose levels of expression change in WT S49 cells incubated with CPT-cAMP for 24 h are listed. The proteins are classified according to the DAVID bioinformatics software. The average ratios (WT CPT 24 h:WT Ctrl) of protein expression are shown for data obtained in at least two independent experiments. The proteins whose levels of expression increased and decreased upon treatment are shown in bold and italics, respectively. Also shown are the number of unique peptides identified for each protein (unique_pep) and the number of PQD spectra (spectrum_num) used for protein quantitation.