Table 4. Effect of Incubation with Fsk and IBMX for 6 h on the Protein Expression of WT S49 Cells^a.

	WT Fsk 6 h:WT ctrl
gene symbol	accession number	average ratio	unique_pep	spectrum_num	protein
Cofactor Binding
Pygb	IPI00229796	2.33	3	11	glycogen phosphorylase, brain form
Idh3g	IPI00109169	1.58	2	22	isocitrate dehydrogenase [NAD] subunit γ
Gsr	IPI00760002	5.80	3	30	cytoplasmic isoform of glutathione reductase
Dbi	IPI00108756	0.37	2	15	acyl-CoA-binding protein
Protein Transport
Copa	IPI00761529	2.07	4	7	coatomer protein complex subunit α
Gdi2	IPI00122565	1.69	3	17	isoform 1 of Rab GDP dissociation inhibitor β
Spnb2	IPI00121892	0.48	11	17	isoform 2 of spectrin β chain, brain 1
Macf1	IPI00663180	0.49	7	10	microtubule-actin cross-linking factor 1 isoform 2
Regulation of Transcription
Anp32a	IPI00314736	1.99	2	30	acidic leucine-rich nuclear phosphoprotein 32 family member A
Hmgb1	IPI00620379	2.83	4	12	14 kDa protein
Morf4l2	IPI00463666	2.21	2	10	mortality factor 4-like protein 2
Supt16h	IPI00120344	2.08	4	8	FACT complex subunit
Ywhah	IPI00227392	0.64	3	38	14-3-3 protein η
Adenyl Nucleotide Binding
Idh3g	IPI00109169	1.58	2	22	isocitrate dehydrogenase [NAD] subunit γ
Gsr	IPI00760002	5.80	3	30	glutathione reductase
Psmc3	IPI00758064	0.30	3	17	proteasome (prosome, macropain) 26S subunit ATPase 3
Psme2	IPI00124225	0.44	3	13	Psme2b-ps proteasome activator complex subunit 2
Lrrc59	IPI00123281	0.46	2	17	leucine-rich repeat-containing protein 59
Cops4	IPI00131871	0.56	2	7	COP9 signalosome complex subunit 4
Ncaph	IPI00224053	0.56	3	20	condensin complex subunit 2
Nol5	IPI00463468	0.58	4	37	nucleolar protein 5
Gripap1	IPI00775775	1.64	3	10	GRIP1-associated protein 1
Dap3	IPI00275050	2.68	2	21	isoform 1 of mitochondrial 28S ribosomal protein S29
Exosc4	IPI00310857	1.73	2	35	exosome complex exonuclease RRP41
Ptges3	IPI00762817	9.12	2	5	prostaglandin E synthase 3
Tsnax	IPI00137296	2.76	2	17	translin-associated protein X

^aProteins whose levels of expression change in WT S49 cells incubated with Fsk and IBMX for 6 h are listed. The proteins are classified according to the DAVID bioinformatics software. The average ratios of protein expression (WT Fsk 6 h:WT ctrl) are shown from data obtained in at least two independent experiments. The proteins whose levels of expression increased and decreased upon incubation with Fsk and IBMX are shown in bold and italics, respectively. Also shown are the number of unique peptides identified for each protein (unique_pep) and the number of PQD spectra (spectrum_num) used for protein quantitation.