Table 4. Effect of Incubation with Fsk and IBMX for 6 h on the Protein Expression of WT S49 Cellsa.
WT Fsk 6 h:WT ctrl |
|||||
---|---|---|---|---|---|
gene symbol | accession number | average ratio | unique_pep | spectrum_num | protein |
Cofactor Binding | |||||
Pygb | IPI00229796 | 2.33 | 3 | 11 | glycogen phosphorylase, brain form |
Idh3g | IPI00109169 | 1.58 | 2 | 22 | isocitrate dehydrogenase [NAD] subunit γ |
Gsr | IPI00760002 | 5.80 | 3 | 30 | cytoplasmic isoform of glutathione reductase |
Dbi | IPI00108756 | 0.37 | 2 | 15 | acyl-CoA-binding protein |
Protein Transport | |||||
Copa | IPI00761529 | 2.07 | 4 | 7 | coatomer protein complex subunit α |
Gdi2 | IPI00122565 | 1.69 | 3 | 17 | isoform 1 of Rab GDP dissociation inhibitor β |
Spnb2 | IPI00121892 | 0.48 | 11 | 17 | isoform 2 of spectrin β chain, brain 1 |
Macf1 | IPI00663180 | 0.49 | 7 | 10 | microtubule-actin cross-linking factor 1 isoform 2 |
Regulation of Transcription | |||||
Anp32a | IPI00314736 | 1.99 | 2 | 30 | acidic leucine-rich nuclear phosphoprotein 32 family member A |
Hmgb1 | IPI00620379 | 2.83 | 4 | 12 | 14 kDa protein |
Morf4l2 | IPI00463666 | 2.21 | 2 | 10 | mortality factor 4-like protein 2 |
Supt16h | IPI00120344 | 2.08 | 4 | 8 | FACT complex subunit |
Ywhah | IPI00227392 | 0.64 | 3 | 38 | 14-3-3 protein η |
Adenyl Nucleotide Binding | |||||
Idh3g | IPI00109169 | 1.58 | 2 | 22 | isocitrate dehydrogenase [NAD] subunit γ |
Gsr | IPI00760002 | 5.80 | 3 | 30 | glutathione reductase |
Psmc3 | IPI00758064 | 0.30 | 3 | 17 | proteasome (prosome, macropain) 26S subunit ATPase 3 |
Psme2 | IPI00124225 | 0.44 | 3 | 13 | Psme2b-ps proteasome activator complex subunit 2 |
Lrrc59 | IPI00123281 | 0.46 | 2 | 17 | leucine-rich repeat-containing protein 59 |
Cops4 | IPI00131871 | 0.56 | 2 | 7 | COP9 signalosome complex subunit 4 |
Ncaph | IPI00224053 | 0.56 | 3 | 20 | condensin complex subunit 2 |
Nol5 | IPI00463468 | 0.58 | 4 | 37 | nucleolar protein 5 |
Gripap1 | IPI00775775 | 1.64 | 3 | 10 | GRIP1-associated protein 1 |
Dap3 | IPI00275050 | 2.68 | 2 | 21 | isoform 1 of mitochondrial 28S ribosomal protein S29 |
Exosc4 | IPI00310857 | 1.73 | 2 | 35 | exosome complex exonuclease RRP41 |
Ptges3 | IPI00762817 | 9.12 | 2 | 5 | prostaglandin E synthase 3 |
Tsnax | IPI00137296 | 2.76 | 2 | 17 | translin-associated protein X |
Proteins whose levels of expression change in WT S49 cells incubated with Fsk and IBMX for 6 h are listed. The proteins are classified according to the DAVID bioinformatics software. The average ratios of protein expression (WT Fsk 6 h:WT ctrl) are shown from data obtained in at least two independent experiments. The proteins whose levels of expression increased and decreased upon incubation with Fsk and IBMX are shown in bold and italics, respectively. Also shown are the number of unique peptides identified for each protein (unique_pep) and the number of PQD spectra (spectrum_num) used for protein quantitation.