Figure 8.

Cytosolic pH (pHc) profile in hyphae of G. margarita and G. intraradices under different culture conditions. A, G. margarita juvenile germ tubes defined as hyphae shorter than one spore diameter (n = 8; ▪) and in several-centimeter-long germ tubes growing in the absence (n = 9; ○) or the presence (n = 12; ▵) of a carrot hairy root. B, Magnification of the same curves for the first 20 μm. C, G. intraradices germ tubes (n = 3; ○) and extraradical hypha growing from a mycorrhizal carrot hairy root laid directly on the solid medium (n = 10; ▪) or laid first on a filter paper (n = 7; ▵). D, Magnification of the same curves for the first 20 μm. G. intraradices cytosolic pH values are estimations using the calibration relationship established with G. margarita. Error bars are 95% confidence intervals (se).