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Profile of cytokines and chemokines induced by rMBP::SSP4 in PBMC. PBMC was stimulated with the protein for 12–96 h, and cytokines and chemokines were measured in cells' culture supernatants by ELISA. Unstimulated cells (open bars), cells stimulated with rMBP::SSP4 (full bars), and cells stimulated with MBP (gray bars). Histograms show values in pg/mL (means ± SD) of three experiments run in duplicate. ∗, ∗∗, ∗∗∗ P < 0.05, 0.001, and 0.0001, respectively, versus unstimulated cells.
