Figure 4.

Effect of rMBP::SSP4 on gene expression of CAMs and TNFRs in PBMCs. RT-PCR analysis of CAMs and TNFRs mRNAs in PBMCs was performed as described (see Section 2). PBMCs were stimulated with the protein for 12–96 h, S/E (nonstimulated). The intensities of each band were quantified and plotted from the gels that are on top of each graph corresponding to the expression of genes. GAPDH was used as a control housekeeping gene. ^{∗∗, ∗∗∗} P < 0.001 and 0.0001, respectively, versus unstimulated cells.