Figure 3.

Establishing iPLA ₂ γ-knockdown INS-1 cell lines. INS-1 cells were transfected with FIV constructs containing inserts that produced either scrambled RNA (control) or siRNA directed against sequences in iPLA₂ γ mRNA (KD1, KD2). The relative iPLA₂ γ expression levels in control INS-1 cells and in the iPLA₂ γ-knockdown (KD) cell lines KD1 and KD2 were assessed by quantitative PCR for mRNA (panel (a)) and by Western blotting analysis for iPLA₂ γ-immunoreactive protein (panel (b)). In panel (a), mean values ± SEM are displayed (n = 4). An asterisk (*) denotes a significant difference (P < 0.05) from the value for control INS-1 cells. The immunoblot in panel (b) is representative of four experiments.