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. Author manuscript; available in PMC: 2012 Nov 21.
Published in final edited form as: J Mol Endocrinol. 2008 Sep 3;41(5):367–377. doi: 10.1677/JME-08-0026

Figure 2.

Figure 2

Figure 2

Cross-phosphorylation between ER and HER2 downstream components in BT and BT/HerR cells. BT and BT/HerR1.0 cells were deprived of steroid for 72h and serum starved for 24h, and then treated with DMSO, 1nM estrogen (E2), 1μM tamoxifen (Tam) for 20 min (A and B) or 24h (C and D). Levels of total (t) and phosphorylated (p) MAPK, Akt and ERα at Ser118 were measured in cellular extracts by immunoblot analysis. β-actin was used as a control for equal loading and transfer. Figures are representative of three experiments. Phosphorylation levels of the experiments were estimated by densitometry, and were calculated as the ratio of pMAPK/tMAPK, pAkt/tAkt, or pER/tER.