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. Author manuscript; available in PMC: 2012 Nov 21.
Published in final edited form as: J Mol Endocrinol. 2008 Sep 3;41(5):367–377. doi: 10.1677/JME-08-0026

Figure 4.

Figure 4

Different recruitment of nuclear receptor coactivator or corepressor on estrogen-responsive pS2 promoter by tamoxifen bound ER in BT and BT/HerR cells. BT and BT/HerR1.0 cells were deprived of steroid for 72h and serum starved for 24h, and then treated with DMSO, 1nM 17β-estradiol (E2), or 1μM tamoxifen (Tam) for 45 minutes. Occupancy of the estrogen-responsive pS2 promoter by ER; the coactivator AIB1; the corepressor NCoR was examined by ChIP assay as indicted in “Materials and Methods”. Relevant pS2 promoter sequences were PCR amplified from complexes immunoprecipitated with each antibody. Input lane shows DNA that was PCR amplified from extracts before immunoprecipitation. Gels are representative of three experiments.