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Triptolide binds DCTPP1. (A) Partially purified DCTPP1. Coomassie-stained PAGE shows dimer, denatured by β-mercaptoethanol (+β-ME), monomer and cleaved GST. (B) Isothermal titration calorimetry shows that triptolide binds DCTPP1 with KD = 44 ± 4 μM, ΔH = –19.98 ± 1.70 kcal/mol, n = 0.601 ± 0.075. Integrated heats for the indicated experiments are shown.
