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(A) Agarose gels show BamHI and NdeI restriction enzyme double digest result of the final synthetic silk gene in pET19k. Lower bands are synthetic silk gene insertions, upper bands are Linearized pET19k vector (5.8kb). (B) SDS-PAGE (polyacrylamide gel electrophoresis) of proteins purified from IMAC column and stained by Biosafe Coomassie.
