Table 2.
The effect of blood volume in the Tempus tubes on RNA quality
| Blood volume (ml) | Yield total RNA (ug) | RNA Integrity Number (RIN) | OD260/280 ratio | OD2602/230 ratio |
|---|---|---|---|---|
| A) Adult blood samples (n = 3)* | ||||
| 3.0 |
7.90 ± 1.50 |
8.20 ± 0.06 |
2.19 ± 0.02 |
2.10 ± 0.23 |
| 2.5 |
3.38 ± 0.90 |
8.13 ± 0.27 |
2.24 ± 0.05 |
0.91 ± 0.46 |
| 2.0 |
2.94 ± 0.91 |
8.40 ± 0.08 |
2.12 ± 0.08 |
1.05 ± 0.53 |
| 1.5 |
1.38 ± 0.59 |
3.53 ± 2.53 |
2.51 ± 0.08 |
0.64 ± 0.32 |
| 1.0 |
0.55 ± 0.50 |
1.13 ± 0.13 |
1.19 ± 0.44 |
0.38 ± 0.38 |
| 0.5 |
0.07 ± 0.03 |
1.00 ± 0.00 |
0.77 ± 0.39 |
0.05 ± 0.05 |
| B) Cord blood samples (n = 2) | ||||
| 3.3 |
79.65 ± 4.76 |
7.15 ± 0.78 |
2.15 ± 0.00 |
2.12 ± 0.00 |
| 3.0 |
74.60 ± 1.23 |
7.50 ± 0.65 |
2.17 ± 0.00 |
2.15 ± 0.01 |
| 2.0 |
44.42 ± 1.91 |
9.10 ± 0.00 |
2.18 ± 0.01 |
2.09 ± 0.01 |
| 1.0 |
33.92 ± 3.13 |
9.45 ± 0.04 |
1.98 ± 0.01 |
1.61 ± 0.02 |
| 0.5 | 4.29 ± 0.11 | 1.03 ± 0.03 | 1.25 ± 0.03 | 0.53 ± 0.01 |
Notes. *There are significant blood volume dependent reductions in the RNA yields and in the RNA qualities (RIN, OD 260/280 and OD 260/230 ratio), p < 0.05. n = number of tubes.