Figure 4.

ABA responsiveness of L. gibba NPR1 LS constructs. The range of each LS construct is diagrammed on a schematic of the NPR1 promoter between −156 and −70 from the transcriptional start. Black boxes indicate the positions of ACGT motifs; the gray box indicates the position of a CE3-like motif. Following bombardment with the designated LS mutant or wild-type NPR1–156 construct, plants were treated with 2 min of R plus water (white bars) or 10 μm ABA (cross-hatched bars) and returned to D for 16 to 18 h before reporter gene activity was assayed. The normalized ratios of reporter LUC to internal standard GUS activities are reported as relative activities; se values are shown. WT, Wild type.