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. 2012 Aug 31;13:441. doi: 10.1186/1471-2164-13-441

Table 1.

Fold change verification of gene expression by RT-PCR

Gene symbol Gene title Microarray fold change
Quantitative PCR fold change
Fasting vs. Fed Ins-Neu vs. Fed Ins-Neu vs. Fasting Fasting vs. Fed Ins-Neu vs. Fed Ins-Neu vs. Fasting
FBXO8
F-box protein 8
4.16***
2.09**
−1.99***
2.59***
2.76***
1.06
DUSP5
Dual specificity phosphatase 5
9.43***
1.05
−8.98***
8.13***
1.69***
−4.82***
BNIP3
BCL2/adenovirus E1B 19 kDa interacting protein 3
2.98***
1.53
−1.95**
3.45***
2.26***
−1.52*
PBX3
Pre-B-cell leukemia homeobox 3
−1.62***
1.07
1.75***
−1.22
1.83***
2.22***
IL10RB
Interleukin 10 receptor, beta
1.74***
1.02
−1.71**
1.76***
1.42*
−1.24
EGR1
Early growth response 1
2.43*
−1.58
−3.86**
2.58***
−1.36
−3.52***
NAB1
NGFI-A binding protein 1 (EGR1 binding protein 1)
2.43***
1.06
−2.29***
1.67**
1.52**
−1.10
PDK4
Pyruvate dehydrogenase kinase, isozyme 4
17.28***
7.06**
−2.45**
18.33***
4.01***
−4.57***
CTSL2
Cathepsin L2
2.09***
1.55*
−1.35*
2.96***
2.053***
−1.44
AGTR1
Angiotensin II receptor, type 1
4.15 ***
2.05*
−2.02**
3.52**
1.72*
−2.05*
SESN1 Sestrin 1 1.86** 1.125 −1.65 1.58* 1.47* 1.07

FDR p-value: * p < 0.05, ** p < 0.005, *** p < 0.0005.