Table 3.
MALDI-TOF MS based species identification of 100 randomly selected Culicoides specimens from the 12 trapping sites1
|
Site |
Culicoides species (no.) {no. confirmed by PCR}3 [%4] |
||||
|---|---|---|---|---|---|
|
(altitude2) |
Morphological group |
Unknown Genetic identification4 | No identification5 | ||
| Obsoletus | Pulicaris | OtherCulicoides | |||
| Novazzano |
C. obsoletus (84) {5} |
C. grisescens II (1) |
C. circumscriptus (4) {1} |
0 |
0 |
| (270) |
C. scoticus (5) |
C. pulicaris (1) |
C. pallidicornis (4) |
|
|
| |
|
C. punctatus (1) |
|
|
|
| |
[91.4] |
[1.9] |
[6.7] |
|
|
| Dittingen |
C. obsoletus (3) |
C. lupicaris (2) |
0 |
0 |
0 |
| (360) |
C. scoticus (95) {6} |
|
|
|
|
| |
[99.9] |
[0.1] |
[0] |
|
|
| Commugny |
C. obsoletus (91) {6} |
0 |
0 |
C. kibunensis (2) |
3 |
| (420) |
C. scoticus (4) |
|
|
|
|
| |
[98.4] |
[0] |
[1.6] |
|
|
| Granges |
C. obsoletus (88) {6} |
C. grisescens II (1) |
0 |
0 |
0 |
| (500) |
C. scoticus (9) |
C. pulicaris (2) |
|
|
|
| |
[100] |
[0] |
[0] |
|
|
| Wädenswil |
C. obsoletus (91) {5} |
C. lupicaris (5) {1} |
C. pallidicornis (1) |
0 |
0 |
| (620) |
C. scoticus (2) |
C. pulicaris (1) |
|
|
|
| |
[95] |
[4.2] |
[0.8] |
|
|
| Mühlethurnen |
C. obsoletus(74) {4} |
C. lupicaris (1) |
0 |
C. segnis (1) |
3 |
| (670) |
C. scoticus (19) {1} |
|
|
|
|
| |
C. dewulfi (2) {1} |
|
|
|
|
| |
[99.0] |
[1.0] |
[0] |
|
|
| Bennau |
C. obsoletus (74) {4} |
C. grisescens II (1) {1} |
C. pallidicornis (1) |
0 |
4 |
| (810) |
C. scoticus (11) {1} |
C. lupicaris (9) |
|
|
|
| |
[92.1] |
[5.6] |
[2.2] |
|
|
| Château d’Oex |
C. obsoletus (91) {6} |
C. lupicaris (1) |
0 |
C. newsteadi (1) |
0 |
| (940) |
C. scoticus (3) |
C. pulicaris (3) |
|
|
|
| |
|
C. punctatus (1) |
|
|
|
| |
[90.6] |
[4.7] |
[4.7] |
|
|
| Chaumont |
C. obsoletus (12) {2} |
C. lupicaris (1) |
0 |
0 |
3 |
| (1,110) |
C. scoticus (75) {3} |
C. pulicaris (1) |
|
|
|
| |
C. chiopterus (6) |
C. punctatus (1) |
|
|
|
| |
C. dewulfi (1) {1} |
|
|
|
|
| |
[94.2] |
[1.9] |
[3.9] |
|
|
| Davos |
C. obsoletus (5) |
C. grisescens I (4) {1} |
C. pallidicornis (1) |
0 |
0 |
| (1,560) |
C. scoticus (2) |
C. grisescens II (87) {5} |
|
|
|
| |
|
C. pulicaris (1) |
|
|
|
| |
[4.2] |
[95.0] |
[0.8] |
|
|
| Sils |
C. obsoletus (4) |
C. deltus (6) {1} |
0 |
C. fascipennis (2) |
0 |
| (1,800) |
C. scoticus (3) |
C. grisescens I (5) |
|
C. reconditus (1) |
|
| |
|
C. grisescens II (75) {5} |
|
|
|
| |
|
C. pulicaris (4) |
|
|
|
| |
[4.5] |
[82.9] |
[12.6] |
|
|
| Juf |
|
C. grisescens I (51) {4} |
0 |
C. fascipennis (3) |
0 |
| (2,130) |
|
C. grisescens II (40) {2} |
|
C. reconditus (2) |
|
| |
|
C. pulicaris (2) |
|
C. segnis-like (2)6 |
|
| [1.5] | [94] | [4.5] | |||
1 Specimens randomly selected from a single catch from the high abundance season (August/September 2010), stored in 70% ethanol for more than 1 year and analysed using a database containing validated biomarker mass sets of 15 Culicoides species [27].
2 Meters above sea level.
3 PCR/sequencing [22] with 6 randomly chosen ( http://www.random.org) specimens/site. Species identification by comparison with GenBank entries (>99% identity).
4 Percentage of Culicoides morphologically classified as belonging to Obsoletus group, Pulicaris group or other Culicoides of the catch from which the specimens were selected for the MALDI-TOF MS analyses.
4 Identified by PCR/sequencing [22].
5 No species identification achievable due to poor quality mass spectra.
6 Best match: 96% identity (403/422) with GenBank entry HQ824509 (Ccxx segnis from Switzerland).