Table 2.
PCR amplification primers used in this study
| Genomic region covered* | Forward primer (5′-3′) | Reverse primer (5′-3′) | Product size | Comment^ |
|---|---|---|---|---|
| chr14:95560431-95560500 |
GGTGCTTGGTTATGAGGTAGTCC |
CCCTCAGATTGTTACCAGCG |
70 bp |
Product includes codons 1705–1709 of DICER1; primer sequences from this study |
| chr14:95557604-95557671 |
CATGTAAATGGCACCAGCAA |
AAGAGGATATTGAAGTTCCAAAGG |
68 bp |
Product includes codons 1810–1813 of DICER1; primer sequences from this study |
| chr14:95560345-95560533 |
CTTCTGCACAAGCTTACGGTTCCA |
CAGCGATGCAAAGATGGTGTTGT |
188 bp |
Product includes codons 1705–1709 of DICER1; primer sequences from
[10]. |
| chr14:95557565-95557759 | TGGACTGCCTGTAAAAGTGG | ACACACCTGCCAGACTGTCTCC | 194 bp | Product includes codons 1810–1813 of DICER1; primer sequences from [10]. |
* Genomic location is based on reference sequence hg19.
^ Amino acid numbering is based on DICER1 reference sequence [GenBank:NM_177438].