Table 1.
Dilution | Measurement No. | Particles/frame | Completed tracks | Mean particle size (nm) | Measured conc.(LP/ml) | Average measured conc. (LP/ml) | Concentration (LP/ml) | Measured factor (MF) | Theoretical factor (TF) | TF/MF |
---|---|---|---|---|---|---|---|---|---|---|
300 |
1 |
91* |
2896 |
115 |
1,52E+09 |
|
|
|
|
|
2 |
79* |
2407 |
119 |
1,27E+09 |
1,4E+09 |
4,2E+11 |
|
|
|
|
500 |
1 |
120* |
4179 |
100 |
1,93E+09 |
|
|
0,7 |
1,7 |
2,3 |
2 |
115* |
3999 |
103 |
1,86E+09 |
1,9E+09 |
9,5E+11 |
|
|
|
|
900 |
1 |
87* |
2621 |
125 |
1,40E+09 |
|
|
1,4 |
1,8 |
1,3 |
2 |
80* |
2253 |
133 |
1,29E+09 |
1,3E+09 |
1,2E+12 |
|
|
|
|
1000 |
1 |
64* |
2004 |
125 |
1,03E+09 |
|
|
|
|
|
2 |
35 |
755 |
143 |
5,69E+08 |
|
|
|
|
|
|
3 |
23 |
575 |
131 |
3,78E+08 |
4,7E+08 |
4,7E+11 |
|
|
|
|
1500 |
1 |
61* |
2005 |
106 |
9,92E+08 |
|
|
0,6 |
1,5 |
2,6 |
2 |
52 |
1688 |
110 |
8,35E+08 |
8,4E+08 |
1,3E+12** |
|
|
|
|
2000 |
1 |
29 |
748 |
130 |
4,71E+08 |
|
|
1,7 |
1,3 |
0,8 |
2 |
33 |
1004 |
109 |
5,38E+08 |
5,0E+08 |
1,0E+12** |
|
|
|
|
3000 |
1 |
13* |
343 |
149 |
2,16E+08 |
|
|
1,3 |
1,5 |
1,2 |
2 |
24 |
640 |
146 |
3,90E+08 |
|
|
|
|
|
|
3 |
24 |
681 |
127 |
3,94E+08 |
3,9E+08 |
1,2E+12** |
|
|
|
|
1,15E+12 | Final concentration (LP/ml) |
* Measurements where the “particle/frame” was higher than 60 or lower than 20 (consequently these measurements were eliminated from final calculation).
**Concentration values with TF/MF factor between 0.8 and 1.2 (these values were used to calculate the original concentration of latex particles).