Table 2.
Inter-day variability of the NTA method
| Sample | Dilution | Measured conc. (VP/ml) | Average measured conc (VP/ml) | Sample conc. (VP/ml) | MF | TF | TF/MF | Conc. (VP/ml) |
|---|---|---|---|---|---|---|---|---|
| Day 1 |
20 |
6,90E+08 |
|
|
|
|
|
|
| 20 |
4,27E+08 |
5,59E+08 |
1,12E+10 |
|
|
|
|
|
| 30 |
4,63E+08 |
|
|
1,3 |
1,5 |
1,1 |
1,2E+10 |
|
| 30 |
3,73E+08 |
4,18E+08 |
1,25E+10 |
|
|
|
|
|
| Day 2 |
20 |
6,08E+08 |
|
|
|
|
|
|
| 20 |
8,12E+08 |
7,10E+08 |
1,42E+10 |
|
|
|
|
|
| 30 |
4,89E+08 |
|
|
1,4 |
1,5 |
1,1 |
1,5E+10 |
|
| 30 |
5,49E+08 |
5,19E+08 |
1,56E+10 |
|
|
|
|
|
| Day 3 |
20 |
6,28E+08 |
|
|
|
|
|
|
| 20 |
6,75E+08 |
6,52E+08 |
1,30E+10 |
|
|
|
|
|
| 30 |
5,50E+08 |
|
|
1,3 |
1,5 |
1,2 |
1,4E+10 |
|
| 30 |
4,60E+08 |
5,05E+08 |
1,52E+10 |
|
|
|
|
|
| Day 4 |
20 |
5,73E+08 |
|
|
|
|
|
|
| 20 |
5,35E+08 |
5,54E+08 |
1,11E+10 |
|
|
|
|
|
| 30 |
3,79E+08 |
|
|
1,5 |
1,5 |
1,0 |
1,1E+10 |
|
| 30 |
3,52E+08 |
3,66E+08 |
1,10E+10 |
|
|
|
|
|
| Average |
1,3E+10 |
|||||||
| STDV |
1,8E+09 |
|||||||
| %STDV | 14,1 | |||||||
Inter-day variability of the NTA method determined by measuring the same sample (Ad5 virus) for 4 consecutive days. MF- the ratio between concentrations of two sample dilutions determined with NTA; TF-the ratio between two dilutions of the same sample analyzed.