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Thermostability of enzyme variants from each generation
| Generation | Variant | t1/2(min, at 60°C) | Fold Improvement |
|---|---|---|---|
| 0 |
parent |
4.0 |
1.0 |
| 1 |
ep 1-1 |
9.8 |
2.4 |
| |
ep 1-26 |
8.7 |
2.2 |
| 2 |
ep 2-3 |
11.3 |
2.8 |
| |
ep 2-4 |
21.7 |
5.4 |
| |
ep 2-8 |
14.4 |
3.6 |
| |
ep 2-12 |
13.5 |
3.4 |
| 3 |
S 3-1 |
38.8 |
9.7 |
| |
S 3-4 |
58.9 |
13.9 |
| |
S 3-18 |
48.0 |
12.0 |
| 4 |
S 4-3 |
184.0 |
46.0 |
| |
S 4-13 |
168.3 |
42.1 |
| S 4-20 | 159.5 | 40.0 |
