Table 4.
Oligonucleotides utilized in this study
| Primer | Oligonucleotide sequence |
|---|---|
| BC-F |
GCTGCTAAAGAAGAAGGGGTATCTCTCGAGAAAAGAGAGGCTGAAGCTTACGTAGAATTCCCTAGG |
| BC-R |
GTAAGTGCCCAACTTGAACTGAGGAACAGTCATGTCTAAGGCGAATTAATTCGCGGCCGC |
| A-F |
GGCCGCGAATTAATTCGCCTTAGACATG |
| A-R |
AGATCTTGATATAAATTTCACGTTTAAAATC |
| D-F |
TCGACAATTGGTTTGACTAATTCCATAATCTG |
| D-R |
ACCTTTCGTCTTTGGATGTTAGTCT |
| BC-F10 |
ATTCCCTAGG |
| BC-R10 |
TCGCGGCCGC |
| BC-F25 |
TGAAGCTTACGTAGAATTCCCTAGG |
| BC-R25 |
CTAAGGCGAATTAATTCGCGGCCGC |
| BC-F40 |
CGAGAAAAGAGAGGCTGAAGCTTACGTAGAATTCCCTAGG |
| BC-R40 |
GAGGAACAGTCATGTCTAAGGCGAATTAATTCGCGGCCGC |
| BC-F55 |
GAAGGGGTATCTCTCGAGAAAAGAGAGGCTGAAGCTTACGTAGAATTCCCTAGG |
| BC-R55 |
GCCCAACTTGAACTGAGGAACAGTCATGTCTAAGGCGAATTAATTCGCGGCCGC |
| BC-F70 |
CATTGCTGCTAAAGAAGAAGGGGTATCTCTCGAGAAAAGAGAGGCTGAAGCTTACGTAGAATTCCCTAGG |
| BC-R70 |
CGGTCTTCTCGTAAGTGCCCAACTTGAACTGAGGAACAGTCATGTCTAAGGCGAATTAATTCGCGGCCGC |
| BC-F85 |
TACTACTATTGCCAGCATTGCTGCTAAAGAAGAAGGGGTATCTCTCGAGAAAAGAGAGGCTGAAGCTTACGTAGAATTCCCTAGG |
| BC-R85 |
AGAATCTAGCAAGACCGGTCTTCTCGTAAGTGCCCAACTTGAACTGAGGAACAGTCATGTCTAAGGCGAATTAATTCGCGGCCGC |
| BC-F65 |
CTGCTAAAGAAGAAGGGGTATCTCTCGAGAAAAGAGAGGCTGAAGCTTACGTAGAATTCCCTAGG |
| BC-R65 | TTCTCGTAAGTGCCCAACTTGAACTGAGGAACAGTCATGTCTAAGGCGAATTAATTCGCGGCCGC |
The number in the name of the primers indicated the length of the overlapping sequences.