Figure 2.

Time-course of protein concentration during fed-batch cultivations. Gene sequences were optimized either by codon pair optimization (CP) [21] or by applying the high CAI codons for methanol induced genes (HM) [11]. Copy numbers (CN) are specified in the legend. Panel A: Expression of differently codon optimized TrCBH2 variants under the control of P(AOX1), P(GAP) or the synthetic promoter P(De) [6]. HM optimized variants (closed symbol), CP optimized variants (open symbol). Panel B: Time-course of TrCBH2 expression normalized to copy number. Legend labeling see panel A. Panel C: Expression of TrbMan (open symbol) and TlXynA (closed symbol) under the control of P(En) [6]. For virtual gels of the protein yields during the fermentation runs please refer to Additional file 1 and the raw data can be found in Additional file 2