Figure 4.

Time-course of protein concentration during fed-batch cultivations. Gene sequences were optimized by applying the high CAI codons for methanol induced genes (HM) [11]. Copy numbers (CN) are specified in the legend. Dotted lines indicate previously obtained results (compare Figure 2). Panel A: TrCBH2 expression under the control of P(AOX1) or the synthetic promoters P(En) or P(De) [6]. Panel B: TrbMan expression under the control of P(De) and P(En). For a visual representation of the protein yields during the fermentation runs please refer to the virtual gels presented in Additional file 1 and the raw data can be found in Additional file 2