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. 2012 Nov 21;7(11):e49987. doi: 10.1371/journal.pone.0049987

Figure 3. NTF3 is a direct target of miR-200c. A.

Figure 3

. Regions of the 3′ UTR where miR-200c is predicted to bind. B. Hec50 cells transfected with NTF3 luciferase constructs and 24 hrs later treated with transfection reagent only (mock), scrambled negative control (neg), miR-200c mimic (200c), miR-200c antagomiR alone (α200c) or in conjunction with miR-200c (α200c+200c) and luciferase assay performed. Columns, mean of five biological replicates, bars, SEM. C. Cells transfected with miRNA constructs and 48 hrs later medium collected for analysis by NTF3 ELISA. Columns, mean of three biological replicates, bars, SEM.