Figure 1 .

Vectors for anti-HER2 light-chain and heavy chain expression. (a) Plasmid pGLY10969 encodes the light chain of anti-HER2 antibody and mCherry red fluorescent protein in association with the arsenic resistance selection marker and targets the URA6 locus in P. pastoris genome (b) Plasmid pGLY10970 encodes the heavy chain of anti-HER2 antibody and yEGFP3 green fluorescent protein in association with nourseothricin resistance selection marker and targets the URA6 locus in P. pastoris genome. (c) Plasmid pGLY6830 encodes both light-chain and heavy-chain of anti-HER2 expression cassettes in association with zeocin resistance selection marker and targets to the TRP2 locus in P. pastoris genome. The SpeI restriction enzyme site used to linearize each vector prior to yeast transformation is underlined.