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Eadie-Hofstee and Michaelis-Menten plots of glucose initial uptake rates in LF and RF.S. cerevisiae PYCC 4072 cells harvested at the indicated incubation times from the nitrogen-limited fermentation (LF) (Figure1) that was either or not supplemented with diammonium phosphate 72 hours after fermentation start (LF + RF), were used to determine the kinetic parameters Km and Vmax shown in Table1.
