Table 1.
List of primary and secondary antibodies used for western blot analysis
| Antibody | Host | Company | Dilution | Duration |
|---|---|---|---|---|
| Anti-rat β-actin |
Mouse |
Chemicon (MAB1501R) |
1:500 |
Overnight 4°C |
| Anti-rat matrix metalloproteinase (MMP)-9 |
Rabbit |
Chemicon (AB19016) |
1:1,000 |
Overnight 4°C |
| Anti-rabbit IgG horseradish peroxidase (HRP) |
Goat |
Jackson Lab (11-035-144) |
1:5,000 |
1 h RT |
| Anti-mouse IgG horseradish peroxidase (HRP) | Goat | Sigma (A-3682) | 1:2,000 | 1 h RT |
Antibody concentrations and incubation conditions are listed.