Table 2.
List of primary and secondary antibodies used for immunohistochemistry
| Antibody | Host | Company | Dilution | Duration |
|---|---|---|---|---|
| Anti-rat ionized calcium binding adapter molecule (Iba)-1 |
Rabbit |
Wako (019–19741) |
1:300 |
Overnight 4°C |
| Anti-rat endothelial cell antibody (RECA-1) |
Mouse |
Serotec (MCA970R) |
1:100 |
Overnight 4°C |
| Anti-human IgG-TRITC |
Goat |
Chemicon (AP504R) |
1:300 |
1 h RT |
| Anti-rabbit IgG AlexaFluor 488 |
Goat |
Invitrogen (A11034) |
1:300 |
1 h RT |
| Anti-mouse IgG AlexaFluor 488 | Goat | Invitrogen (A11001) | 1:300 | 1 h RT |
Antibody concentrations and incubation conditions are listed.