In situhybridization expression ofClSHP1,ClSHP2, andClALCduringCakile lanceolatacarpel development. (a, b) Transverse section through an old bud showed ClSHP1 expression in the ovules of (a) distal and (b) proximal segments. (c) ClSHP1 signal observed in the ovules, septum, and nectaries of an old bud, longitudinal section. (d) ClSHP2 signal was observed in the developing placental tissue. (e-g) ClSHP2 expression was observed in the ovules and septa of (e) distal and (f) proximal segments, transverse sections, and in longitudinal (g). (h, i) Transverse section through an older bud showed weak ClALC expression in septum, ovules, and funiculus in (h) proximal and (i) distal segments. (j) ClALC sense probe of an older bud. Arrows indicate the position of the joint. o, ovules; ow, ovary walls; r, replum; s, septum; v, valves. Scale bar: 100 μm (a, b, d, e, f, h, i, j), 500 μm (c, g).