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Effect of temperature and benzyl alcohol (BA) on soluble D-PhgAT production. SDS-PAGE analysis of soluble (S) and insoluble (I) protein fractions from P. pastoris KM71 expressing D-PhgAT under the control of AOX1 promoter after induction for 24 h at 30°C, 25°C, and at 20°C without and with 0.4 mM benzyl alcohol added to the culture. Lane M, molecular mass markers. The arrow indicates protein bands of D-PhgAT.
