Table 1.
Copy number of dpgA and groEL-groESgenes and D-PhgAT activity of different P. pastoris clones
|
Strain name |
Host strain |
Integrated plasmid |
Integration site |
Copy number |
Specific D-PhgAT activity [U·mg-1]a |
Volumetric D-PhgAT activity [U·L-1]b |
|
|---|---|---|---|---|---|---|---|
| dpgA | groEL-groES | ||||||
| KM_AT3 |
KM71 |
pPIC3.5K_ |
HIS4 |
3 |
0 |
0.0054 |
2.9 |
| |
|
D-PhgAT |
|
|
|
|
|
| KM_AT1_ELS1 |
KM71 |
pAO_ |
HIS4 |
1 |
1 |
12.6 |
7531 |
| |
|
D-PhgAT_GAP _ELS |
|
|
|
|
|
| KM_AT3_ELS3 |
KM71_D-PhgAT |
pPICZ_26S_GAP_ELS |
5′AOX1 |
3 |
3 |
23.3 |
14558 |
| KM_AT3_ELS4 |
|
|
5′AOX1 |
3 |
4 |
45.3 |
34809 |
| KM_AT3_ELS2 |
|
|
26S rDNA |
3 |
2 |
14.6 |
11027 |
| KM_AT3_ELS10 | 26S rDNA | 3 | 10 | 60.1 | 41730 | ||
Cells were cultured at 30°C.
a Specific activity was calculated as unit of D-PhgAT activity per mg of soluble cellular protein.
b Volumetric activity was calculated as unit of D-PhgAT activity obtained per liter of P. pastoris culture.