. 2012 Apr 19;11:47. doi: 10.1186/1475-2859-11-47

Table 3.

Primers used in PCR and DNA sequencing reactions. F: forward primer, R: reverse primer, underlined: restriction endonuclease site

Name	Sequence (5^′-3^′)	Application
FwtdpgA	`CG`GGATCC`ACCATGTCGATCCTTAACG`	Amplifying and cloning
RwtdpgA	`AG`GAATTC`TCATGATTGGTTTCCAGAC`
FdpgA_3.5 K	`CG`GGATCC`ACCATGTCCATCCTGAACG`
RdpgA_3.5 K	`AG`GAATTC`TTAGCAGTGATGGTGAT`
FdpgA_AO	`G`GAATTC`ACCATGTCCATCCTGAACGACTACAAGAG`
RdpgA_AO	`CA`GGTCTCCAATT`GTTAGCAGTGATGGTGATGGTGATG`
FgroES_GAP	`G`GAATTC`ACCATGAATATTCGTCCATTGC`
RgroES_GAP	`GC`TCTAGA`TTACGCTTCAACAATTGCCAG`
FgroEL_GAP	`G`GAATTC`ACCATGGCAGCTAAAGACGTAAAATTCGGTAACG`
RgroEL_GAP	`GC`TCTAGA`TTACATCATGCCGCCCATGCCACC`
FgroES_AO	`G`GAATTC`ACCATGAATATTCGTCCATTGC`
RgroES_AO	`CA`GGTCTCCAATT`GTTACGCTTCAACAATTGCCAG`
FgroEL_AO	`G`GAATTC`ACCATGGCAGCTAAAGACGTAAAATTCGGTAACG`
RgroEL_AO	`CA`GGTCTCCAATT`GTTACATCATGCCGCCCATGCCACC`
F_ELS_PICZ	`CCG`CTCGAG`TTTTGGTCATGCATGAGATC`
R_ELS_PICZ	`ATAGTTTA`GCGGCCGC`TTTTGAAGCTATGGTGTG`
F_GroEL	`TGTCCGTACCATGCTCTGAC`	Copy number determination
R_GroEL	`CAGGTAGCCACGGTCGAAC`
F_D-PhgAT	`GCCTGCTCCAGGTGTCTTGC`
R_D-PhgAT	`ACTGTCTAGCCAATTCAGCACC`
F_Actin	`AAAAGATCTGGCACCACACC`
R_Actin	`AGTGGTTCTACCGGAAGCG`
5^′AOX1	`GACTGGTTCCAATTGACAAGC`	DNA sequencing
3^′AOX1	`GCAAATGGCATTCTGACATCC`