Figure 8. Prx1 Activation of ERK and eIF-4E is Required for HIF-1α Induction.

A/B. 2H-11 cells were treated with DMSO or U0126 (MEKI) prior to Prx1 stimulation. C. Cell lysates were generated and total eIF-4E was isolated by immunoprecipitation. Fractions were separated by electrophoresis and stained for expression of phosphorylated or total eIF-4E. A representative blot is shown; quantization of relative peIF-4E expression from 3 experiments is shown below; error bars represent SEM, n = 3. D. Cell lysates were generated and a Western blot was performed with antibodies specific to HIF-1α and GAPDH. Quantization of relative HIF-1α expression from 3 experiments is shown; error bars represent SEM. ** Represents P≤0.01 when compared to control untreated levels; ## represents P≤0.01 when compared to control stimulated values.