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SuSy protein levels in roots from the Sh1Sus1 homozygous wild type, the sh1Sus1 single mutant, and the sh1sus1 double mutant. Root-tip extracts containing 20 μg of protein were analyzed by SDS-PAGE. Detection of SuSy protein on immunoblots was carried out using a polyclonal antiserum that detected both SuSy subunits. The molecular mass (92 kD) of the SS1 subunit is indicated to the right of the digitized image of the original gels.
