Table II.
In vitro enzyme activities in root extracts from NHPT and HPT seedlings of the Sh1Sus1 and the sh1sus1 genotype
| Genotype and Treatment | Enzyme Activity
|
Protein | ||||
|---|---|---|---|---|---|---|
| SuSy | INV
|
HK | FK | |||
| Neutral | Acid | |||||
| nmol h−1 tip−1 | μg tip−1 | |||||
| Sh1Sus1 | ||||||
| NHPT | 155 ± 27 | 162 ± 13 | 1046 ± 68 | 34 ± 3 | 68 ± 14 | 61 ± 1 |
| HPT | 326 ± 10 | 86 ± 14 | 202 ± 19 | 106 ± 14 | 82 ± 14 | 64 ± 1 |
| sh1sus1 | ||||||
| NHPT | nda | 123 ± 8 | 459 ± 25 | 23 ± 2 | 49 ± 2 | 40 ± 3 |
| HPT | 19 ± 6 | 67 ± 2 | 100 ± 6 | 77 ± 4 | 48 ± 8 | 38 ± 2 |
Groups of 30 excised root tips from NHPT or HPT seedlings were homogenized for measurement of enzyme activities as described in Methods. Data are means ± sd of three independent repetitions.
nd, Not detectable.