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. 2012 Nov 21;7(11):e50560. doi: 10.1371/journal.pone.0050560

Figure 4. Persistence of L. pneumophila mutants in a K. pneumoniae biofilm.

Figure 4

A base biofilm of K. pneumoniae strain DMDS 92-08-28a pre-formed on stainless steel coupons was inoculated in (A) with either L. pneumophila wild-type strain 130b (▪), flaA mutant NU347(pMMB2002) (♦), or pilQ mutant NU278(pMMB2002) (▴) and in (B) with either L. pneumophila wild-type strain 130b (▪) or bbcB mutant NU388 (•) on day 3 and flow of 1∶100 R2A began at 1–2 ml/min. Each data point represents the averages and standard deviations of colony counts from a single rod, and the experiments presented are representative of two repeats.