Figure 1. EGF+bFGF induce expansion of NSCs as neurospheres in culture.

Brain cells isolated from newborn mice were cultured in 12 well tissue culture plates in NBM+B27 with EGF+bFGF. Neurospheres formed in 7–10 days were photographed under microscope (100×) (A). Neurospheres attach and spread after 10 days in culture (200×) (B). NSCs dissociated from 10 day old neurospheres (200×) (C) were cultured in 96 well tissue culture plates (1×10⁴/0.2 ml/well) and the proliferation was measured by ³H thymidine uptake assay (D). Values are means of triplicates±SD and the p values are expressed as *(p<0.05), **(p<0.01) and ***(p<0.001). The figure is a representative of three independent experiments.