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. 2012 Nov 21;7(11):e50500. doi: 10.1371/journal.pone.0050500

Figure 4. PPARγ agonists induce the expression of oligodendrocyte markers in three days in NSCs.

Figure 4

Neurospheres were cultured in poly-D-lysine coated 8 well chamber slides in NBM+B27 with 10 ng/ml EGF+bFGF in the presence of 0 (DMSO) or 1 µM ciglitazone, 15d-PGJ2 or ATRA. After 3 days the cells were stained with GFAP, βIII tubulin, and O4 antibodies along with DAPI and photographed (200×) under fluorescence microscope. The figure is a representative of three independent experiments. The values are mean±SEM and the p values are expressed as *(p<0.05), **(p<0.01), and ***(p<0.001). The figure is a representative of three independent experiments.