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. 2012 Nov 21;7(11):e50500. doi: 10.1371/journal.pone.0050500

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© 2012 Kanakasabai et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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NSCs were cultured in NBM+B27 with 10 ng/ml EGF+bFGF in the presence of 0 or 1 µM ciglitazone, 15d-PGJ2 or ATRA for 3 days and the stem cell gene expression analyzed by qRT-PCR. (A) Heat map showing the expression levels of stemness and differentiation genes in NSCs treated with agonists compared to control. (B) Box plots showing the C_T values of differentiation (Red) and stemness (Green) genes in NSCs treated with agonists compared to control. (C) Scatter plots showing ΔC_T values of differentiation (Red) and stemness (Green) genes in NSCs treated with agonists compared to control. (D) Number of stemness and differentiation genes altered is presented as Venn diagram. The figure is representative of two independent experiments.