Figure 3. A time course study showing pandemic OK/09 impairs RIG-I and IFN-β antiviral immune responses compared to OK/06 and PR8 in human lung.

Human lung slices were exposed to 6×10⁶ PFU/ml of PR8 or OK/09 for 4 h, 8 h, 12 h, 16 h, 24 h, and 32 h. Total RNA was then isolated from lung slices. Quantitative RT-PCR was performed using 100 ng sample RNA and SYBR Green (Quanta Biosciences) in a BIO-RAD iCycler IQ instrument (Hercules, CA). Transcript levels of RIG-1 (A) and IFN-β (B) were normalized relative to the constitutively expressed β-actin gene. Data are from 3 separate experiments. *, p<0.05 for PR8 to OK09. #, p<0.05 for OK/06 to OK/09.